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Fixation

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Dry Fixation

Necessary on smears from blood, bone marrow, and bronchoalveolar lavage provided for Giemsa stain.
After drying by air the smears may be postfixed in methanol
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Wet Fixation

If cytological smears are provided for Papanicolaou stain or H&E, they must immediately be fixed by one of the following methods. The highest nuclear staining quality, however, is achieved with Delaunay’s solution. Between smearing the cells on the slide and Fixation must not elapse more then 1 – 5 seconds!

Qick-Staining Papanicolaou

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1. Smears fixed in Delaunay’s solution
2. Descending alcohol concentrations
3. (96%, 80%, 60%, 50%, destilled water)
4. Haematoxylin*                                                                                                     1.5 min
5. Blueing in water of 20 – 30°C                                                                          ca 15 sec
6. EA 50*       5 – 7 times wave
7. 96% alcohol I + II
8. Xylene

Cover glass

In each alcohol solution move slide 20 – 30 times gently up and down. At the change from one to the next concentration the fluid must evenly run off and not in streaks.

*Recipe see guideline for Papanicolaou stain

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2004-11-29                                                                                                Peter Dalquen/Basel

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Preparing Smears

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Quality of smears depends on

• Cellularity (amount of cells on a slide)
• Even distribution of the cells on the slide
• Cells lying on one level (‚monolayer’), which is difficult to achieve, when the cells are mixed up with viscous secretion, blood or fibrin.

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On Clinical Information

Necessary for Pathologic Diagnosis

Surgical specimens:

Relevant details of patient history clinical findings

Results of special investigations such as x-ray

(image of x-ray can be included)

Gynecological specimens:

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date of last menstrual period

Medicine:

Relevant patient history and clinical findings

Relevant laboratory data such as blood count in hematologic cases

Liver function for liver biopsies

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